Source: Streptomyces achromogenes

5’-G A G C T C-3’
3’-C T C G A G-5’

Reaction Temperature: 37°C

Prototype: SacI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 μg of Lambda/HindIII DNA in 1 hr. Total reaction volume is 50 μl. Enzyme activity was determined in the recommended reaction buffer.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.