Source: Serratia marcescens

5′-C C C G G G-3′
3′-G G G C C C-5′

Reaction Temperature: 25°C

Prototype: SmaI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 μg of Lambda/HindIII in 1 hr. Total reaction volume is 50 μl. Enzyme activity was determined in the recommended reaction buffer.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.