Source: Thermus aquaticus
5’-T C G A-3’
3’-A G C T-5’
Reaction Temperature: 65°C
Inactivation Temperature (20 min): —
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of unmethylated Lambda DNA in 1 hr in a total reaction volume of 50 µl.
Storage Conditions: Store at –20°C.
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.