Source: Thermus aquaticus

5’-G A C C G A (N)₁₁–3’
3’-C T G G C T (N) ₉ -5’

Reaction Temperature: 65°C

Prototype: TaqII

Inactivation Temperature (20 min): —

Reaction Buffer:

1x ONE Buffer

Note 1: It is required to puryfy DNA before digestion. We recommend PCR / DNA Clean-Up Purification Kit or Agarose-Out DNA Purification Kit.

Note 2: over 1 hr digestion is higly recommended. Best results are obtained with 3 hr digestion.

Unit Definition: One unit is the amount of enzyme required to digest 1 µg of pBR322 DNA to obtain stable digestion pattern in 1 hr in a total reaction volume of 50 µl.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.