Color Taq PCR Master Mix (2x) is a ready-to-use solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 , dNTPs and two gel tracking dyes. Use of Color Taq PCR Master Mix (2x) allows to save time and reduce contamination risk due to fewer pipetting steps during PCR set-up. Taq DNA Polymerase is a thermostable enzyme from Thermus aquaticus. The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C. Catalyzes the polymerization of nucleotides into duplex DNA in the 5′→3′ direction in the presence of magnesium ions. Maintains the 5′→3′ exonuclease activity and lacks the 3′→5′ exonuclease activity. Adds extra A at the 3′ ends. Color Taq PCR Master Mix (2x) is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.