OptiTaq DNA Polymerase is a modified and optimized thermostable enzymes blend containing Thermus aquaticus DNA polymerase, Pyrococcus furiosus DNA polymerase and enhancing factors capable of generating over 20 kb products with high fidelity, suitable for applications requiring high temperature synthesis of DNA. Ultrapure, recombinant enzymes are used to prepare OptiTaq DNA Polymerase. The enzymes blend exhibits the 3´→5´ proofreading activity, resulting in considerably higher PCR fidelity than possible with unmodified Taq DNA polymerase. Enables increased amplification product yield in comparison with Taq DNA polymerase over wide range of PCR products. Maintains the 5’´->3´ exonuclease activity. Adds extra A at the 3′ ends. Suitable for multiplex PCR as it exhibits wider tolerance for Mg2+, salts concentration and pH. Improves PCR results with critical templates, such as containing GC-rich regions, palindromes or multiple repeats.
OptiTaq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 20 kb.