Color OptiTaq DNA Polymerase is a modified and optimized thermostable enzymes blend containing Thermus aquaticus DNA polymerase and Pyrococcus sp. DNA polymerase capable of generating PCR products up to 20 kb with high fidelity, suitable for applications requiring high temperature synthesis of DNA. The enzyme is supplemented with two inert gel tracking dyes. Ultrapure, recombinant enzymes are used to prepare OptiTaq DNA Polymerase. The enzymes blend exhibits the 3´->5´ proofreading activity, resulting in considerably higher PCR fidelity and processivity than possible with unmodified Taq DNA polymerase. OptiTaq DNA Polymerase enables increased amplification product yield in comparison with Taq DNA polymerase over wide range of PCR products. Suitable for multiplex PCR as it exhibits wider tolerance for Mg2+, salts concentration and pH. Recommended to use with critical templates, such as containing GC-rich regions, palindromes or multiple repeats. Color OptiTaq DNA Polymerase is recommended to use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 20 kb.
Use of Color OptiTaq DNA Polymerase offers several advantages: visualizes the addition of the polymerase to the reaction, confirms complete mixing, enables direct loading of PCR products onto an agarose gel without addition of a gel loading buffer. The added dyes allow to track electrophoresis progress, do not affect PCR performance, do not interfere with most downstream applications (exeption: the polymerase is not recommended for any downstream application using absorbance or fluorescence excitation).