Ultrapure, recombinant Taq DNA Polymerase is reversibly complexed with anti-Taq monoclonal antibody that blocks replication activity of the enzyme at moderate temperatures. Our carefully selected anti-Taq antibodies have high thermal stability, providing protection against non-specific primer extension from room temperature to 70°C. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94-95°C for two minutes. Formation of complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic “hot start” PCR, which allows for the assembly of PCR reactions at room temperature. High stability of the complexes allows for the enormous increase of PCR specificity, sensitivity and yield in comparison to the conventional PCR assembly method. Automatic “hot start” PCR provides a fast and convenient method when assembling multiple PCR reactions. Both increased specificity and reduced mispriming improve multiplex PCR. Eliminated risk of template cross-contamination and assured safe laboratory practice, due to removed necessity to open hot tubes. Color Perpetual Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.
Use of Color Perpetual Taq DNA Polymerase offers several advantages: visualizes the addition of the polymerase to the reaction, confirms complete mixing, enables direct loading of PCR products onto an agarose gel without addition of a gel loading buffer. The added dyes allow to track electrophoresis progress and do not affect PCR performance, do not interfere with most downstream applications (exeption: the polymerase is not recommended for any downstream application using absorbance or fluorescence excitation).