onTaq DNA Polymerase is a modified “hot start” enzyme which is blocked at moderate temperatures and allows room temperature reaction setup. The polymerase activity is restored during 10 min initial denaturation step. Use of the onTaq DNA Polymerase allows for the increase of PCR specificity, sensitivity and yield in comparison to the conventional PCR assembly method. Both increased specificity and reduced mispriming improve multiplex PCR. Thermostable onTaqDNAPolymerase replicates DNA at 72°C and exhibits a half-life of 40 min at 95°C. Catalyzes the polymerization of nucleotides into duplex DNA in the 5′→3′ direction in the presence of magnesium ions. Contains the 5′→3′ exonuclease activity. Lacks the 3′→5′ exonuclease activity. onTaq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.
onTaq DNA polymerase is sold at a concentration of 2.5 u / μl.