Perpetual Taq PCR Master Mix (2x) is a ready-to-use solution containing Perpetual Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs. Use of Perpetual Taq PCR Master Mix (2x) allows to save time and reduce contamination risk due to fewer pipetting steps during PCR setup. Perpetual Taq DNA Polymerase contains recombinant Taq DNA Polymerase bound to an anti-Taq monoclonal antibody that blocks polymerase activity at moderate temperatures. Anti-Taq antibodies inhibit polymerase activity at temperatures up to 70°C. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94-95°C for two minutes. Formation of complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic “hot start” PCR, which allows for convenient room-temperature reaction setup. “Hot start” PCR may increase specificity, sensitivity and yield of a PCR reaction in comparison to the conventional PCR assembly method. Perpetual TaqDNA Polymerase replicates DNA at 72°C and exhibits a half-life of 40 min at 95°C. Perpetual Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.