Description:

• Hybrid PCR Master Mix (2x) is a ready-to-use solution containing Hybrid DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs.
• Use of Hybrid PCR Master Mix (2x) allows to save time and reduce contamination risk due to fewer pipetting steps during PCR set-up.
• Hybrid is a genetically engineered thermophilic DNA polymerase.
• Ultrapure recombinant enzyme.
• The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5’→3′ direction in the presence of magnesium ions.
• The enzyme exhibits the 3’→5′ proofreading activity, resulting in 50-fold higher PCR fidelity than possible with Taq DNA Polymerases.
• The enzyme generates blunt ends.
• Enhanced polymerase processivity allows to use shorter extension times. 
• Hybrid DNA Polymerase has enhanced target length capability with regard to genomic targets (up to 12 kb from human genomic DNA).
• Due to the polymerase modification, the optimal reaction conditions (especially annealing temperatures) differ from standard PCR protocols.
• Hybrid PCR Master Mix (2x) is recommended for general use in PCR, use in high-fidelity PCR, PCR of GC-rich sequences or problematic secondary structures and cloning of blunt-ended PCR products.