Hybrid PCR Master Mix (2x)

nr kat. E2750

Description:

  • Hybrid PCR Master Mix (2x) is a ready-to-use solution containing Hybrid DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs.
  • Use of Hybrid PCR Master Mix (2x) allows to save time and reduce contamination risk due to fewer pipetting steps during PCR set-up.
  • Hybrid is a genetically engineered thermophilic DNA polymerase.
  • Ultrapure recombinant enzyme.
  • The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5’→3′ direction in the presence of magnesium ions.
  • The enzyme exhibits the 3’→5′ proofreading activity, resulting in 50-fold higher PCR fidelity than possible with Taq DNA Polymerases.
  • The enzyme generates blunt ends.
  • Enhanced polymerase processivity allows to use shorter extension times. 
  • The modification of Hybrid DNA Polymerase enhances target length capability of PfuPlus! DNA Polymerase with regard to genomic targets (up to 12 kb from human genomic DNA).
  • Due to the polymerase modification, the optimal reaction conditions (especially annealing temperatures) differ from standard PCR protocols.
  • Hybrid PCR Master Mix (2x) is recommended for general use in PCR, use in high-fidelity PCR, PCR of GC-rich sequences or problematic secondary structures and cloning of blunt-ended PCR products.