Hybrid DNA Polymerase

cat. no. E2950

Hybrid is a genetically engineered proofreading thermostable polymerase DNA . The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5′->3′ direction in the presence of magnesium ions and and generates blunt ends.. The enzyme exhibits the 3′->5′ proofreading activity, resulting in over 10-fold higher PCR fidelity than possible with Taq DNA Polymerases. Enhanced polymerase processivity allows to use shorter extension times.  Hybrid DNA Polymerase has enhanced target length capability with regard to genomic targets (up to 12 kb from human genomic DNA).

Due to the genetic modification of the polymerase, the optimal reaction conditions (especially annealing temperatures) differ from standard PCR protocols.

Hybrid DNA Polymerase is recommended for general use in PCR, use in high-fidelity PCR, PCR of GC-rich sequences or problematic secondary structures and cloning of blunt-ended PCR products.