GeneMAGNET PCR / DNA Clean-Up Purification Kit is fast cleanup of DNA fragments from PCR and other enzymatic reactions.
Fragment of sizes from approximately 100 bp to over 15 kb can be obtained in ultrapure form. Contaminants such as: ethidum bromide, primers (below 40 nt), short double-stranded DNA (below 20 bp), RNA, Taq DNA Polymerase, Pfu DNA Polymerase, endo- and exonucleases, DNA-binding and modifying proteins, BSA and other enzymes/proteins, lipids, endotoxins, dyes, detergents, nucleotides, radio- and chemical labels, EDTA, problematic restriction and ligation inhibitors, buffers and salts are effectively removed. Coloured binding buffer is very helpful in simultaneous processing of multiple samples. DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.
GeneMAGNET line is based on the use of silica paramagnetic beads (Magnetic Beads) for selective binding of RNA and DNA. The use of specially designed binding and washing buffers enables the efficient purification of highly pure nucleic acids.