GeneMAGNET Soil DNA Purification Kit is magnetic beads based kit for the isolation of total DNA from environmental samples: soil, sediment, compost, manure, stool and water samples. Purified DNA is free of contaminants, such as: humic substances, proteins, lipids, dyes, detergents, buffers or salts.
The sample is added to a tube containing glass beads and the lysis buffer. DNA is released into the solution during shaking. Next, humic substances that strongly inhibit downstream applications, are precipitated by PR buffer. DNA bind to magnetic beads suspended in the binding buffer. Contaminants are removed from the samples in three wash steps. High‑quality cellular DNA is then eluted in low salt buffer. Isolated DNA is ready for downstream applications without the need for the ethanol precipitation.
GeneMAGNET line is based on the use of silica paramagnetic beads (Magnetic Beads) for selective binding of RNA and DNA. The use of specially designed binding and washing buffers enables the efficient purification of highly pure nucleic acids.