GeneMAGNET Human and Animal Tissue DNA Purification Kit

cat. no. E3425

GeneMAGNET Human and Animal Tissue DNA Purification Kit is designed for rapid manual or automatic purification of total DNA (genomic, mitochondrial) from a variety of tissues and biological liquids. Purified DNA is free of contaminants, such as: RNA, proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others.

Tissues and cells are desintegraded in the presence of lysis buffers combination. Further, Proteinase K digests cellular proteins, including DNA binding proteins, among them nucleases. Optimized buffer and ethanol are added to provide selective conditions for DNA binding to Magnetic Beads. Traces of contaminants remaining in the solution are efficiently removed in three wash steps. High-quality cellular DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.

GeneMAGNET line is based on the use of silica paramagnetic beads (Magnetic Beads) for selective binding of RNA and DNA. The use of specially designed binding and washing buffers enables the efficient purification of highly pure nucleic acids.