GeneMAGNET Food DNA Purification Kit is designed for rapid purification of DNA from raw or processed food of plant, animal or mixed origin by manual or automatic method and can be used for the identification of DNA from Genetically Modified Organisms (GMO) in the food. Purified DNA is free of contaminants, such as: proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others.
Sample is finely grinded and lysed in the presence of buffer preserving the integrity of even traces of DNA. Further, Proteinase K digests contaminating proteins, including nucleases. Specialized solution is added to precipitate enzyme inhibitors that strongly inhibit downstream applications. Optimized buffer and ethanol provide selective conditions for DNA binding to the silica covered magnetic beads. Contaminants are efficiently removed in three wash steps. High-quality cellular DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE. Isolated DNA is ready for downstream applications without the need for the ethanol precipitation.
GeneMAGNET Food DNA Purification Kit cat. no E3427 is magnetic beads-based kit designed for manual or automatic DNA isolation from raw or processed food derived from plant, animal or mixed origin.
|Fig. 1. Comparable PCR reaction results with Color Taq PCR Master Mix (2x) cat. no E2525 on DNA isolated with spin-columns (MATRIX) and Magnetic Beads (MAGNET) Food DNA Purification Kits using universal plant detection PCR primers. ctrl+ positive control, ctrl- negative control. MM – Perfect 100-1000 bp DNA Ladder (cat. no E3141).|
|Fig. 2. Very good amplification efficiency with qPCR Fast SG qPCR Master Mix (2x) cat. no E0411 on DNA purified from soya meal with Magnetic Beads by GeneMAGNET Food DNA Purification Kit vs. spin-column based GeneMATRIX Food-Extract DNA Purification Kit (cat. no E3525) using universal plant detection PCR primers.
GeneMATRIX Reaction efficiency: 93.45 %,
|Fig. 3. Mammal gene MT-CYB detection with qPCR Fast SG qPCR Master Mix (2x) cat. no E0411 on DNA isolated from wiener (sausage) with GeneMAGNET Food DNA Purification Kit.|
GeneMAGNET Food DNA Purification Kit:
– high DNA isolation efficiency from foods
– DNA free from PCR/qPCR inhibitors
GeneMAGNET line is based on the use of silica paramagnetic beads (Magnetic Beads) for selective binding of RNA and DNA. The use of specially designed binding and washing buffers enables the efficient purification of highly pure nucleic acids.