GeneMAGNET Food DNA Purification Kit is designed for rapid purification of DNA from raw or processed food of plant, animal or mixed origin by manual or automatic method and can be used for the identification of DNA from Genetically Modified Organisms (GMO) in the food. Purified DNA is free of contaminants, such as: proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others.
Sample is finely grinded and lysed in the presence of buffer preserving the integrity of even traces of DNA. Further, Proteinase K digests contaminating proteins, including nucleases. Specialized solution is added to precipitate enzyme inhibitors that strongly inhibit downstream applications. Optimized buffer and ethanol provide selective conditions for DNA binding to the silica covered magnetic beads. Contaminants are efficiently removed in three wash steps. High-quality cellular DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE. Isolated DNA is ready for downstream applications without the need for the ethanol precipitation.
GeneMAGNET line is based on the use of silica paramagnetic beads (Magnetic Beads) for selective binding of RNA and DNA. The use of specially designed binding and washing buffers enables the efficient purification of highly pure nucleic acids.