Swab-Extract DNA Purification Kit

cat. no. E3530

GeneMATRIX Swab-Extract DNA Purification Kit is designed for rapid purification of total DNA (genomic, mitochondrial) from the swabs taken from mucous membranes, semen, saliva or blood, among others. Purified DNA is free of contaminants, such as: proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others. Both freshly taken and dried samples are subjected to lysis in the presence of special tissue- and cells solubilizing buffer, aided by proteolysis. Further, Proteinase K digests contaminating proteins, including stripping-off DNA of all bound proteins, among them nucleases. Optimized buffer and ethanol are added to provide selective conditions for DNA binding during brief centrifugation, while contaminants pass through the GeneMATRIX resin in the spin-column. Traces of contaminants remaining on the resin are efficiently removed in two wash steps. High-quality cellular DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.