Universal RNA Clean Up Kit removes DNA and other contaminants from RNA samples isolated from different sources (animal and plant tissues, bacteria, yeast, blood etc.).
RNA can be purified by phenol-chloroform extraction, spin column or the use of magnetic beads. Nevertheless it is usually contaminated by genomic DNA which affects downstream applications like RT-qPCR or RNAseq. In the first step DNase I and a reaction buffer are added to the RNA sample in order to digest DNA. Then the nucleic acid binding buffer is added to the sample. This buffer inactivates any RNases/DNases present in the sample. During short centrifugation RNA binds selectively to the membrane. Unbound impurities remain in the column flow-through. Traces of contaminants remaining on the membrane are efficiently removed in two wash steps.
The elution of purified RNA is carried out with RNase-free water. Purified nucleic acids have a length of over 25 nt (miRNA) and are free of proteins, nucleases, other impurities and are ready for use in amplification reactions or storage at -20°C.
The use of unique chemical composition of the matrices, in combination with optimized spin-column design and buffers enables the RNA recovery up to 100 %.
|DNR II||1.5 ml||6 ml||2-8°C|
|DNase I (5 U / 1 μl)||275 U||1100 U||-20°C|
|DNase I buffer||100 μl||300 μl||15-25°C|
|RL||12 ml||48 ml||15-25°C|
|Wash RNA||27 ml||108 ml||15-25°C|
|RNase-free water||3 ml||12 ml||15-25°C|
|RNA Binding Columns||25 szt.||2 x 50 szt||2-8°C|