Universal RNA Clean-Up Kit removes DNA and other contaminants from RNA samples isolated from different sources (animal and plant tissues, bacteria, yeast, blood etc.).
RNA can be purified by phenol-chloroform extraction, spin column or the use of magnetic beads. Nevertheless it is usually contaminated by genomic DNA which affects downstream applications like RT-qPCR or RNAseq. In the first step DNase I and a reaction buffer are added to the RNA sample in order to digest DNA. Then the nucleic acid binding buffer is added to the sample. This buffer inactivates any RNases/DNases present in the sample. During short centrifugation RNA binds selectively to the membrane. Unbound impurities remain in the column flow-through. Traces of contaminants remaining on the membrane are efficiently removed in two wash steps.
The elution of purified RNA is carried out with RNase-free water. Purified nucleic acids have a length of over 25 nt (miRNA) and are free of proteins, nucleases, other impurities and are ready for use in amplification reactions or storage at -20°C.
The use of unique chemical composition of the matrices, in combination with optimized spin-column design and buffers enables the RNA recovery up to 100 %.
|DNR II||1.5 ml||6 ml||2-8°C|
|DNase I (5 U / 1 μl)||275 U||1100 U||-20°C|
|DNase I buffer||100 μl||300 μl||15-25°C|
|RL||12 ml||48 ml||15-25°C|
|Wash RNA||27 ml||108 ml||15-25°C|
|RNase-free water||3 ml||12 ml||15-25°C|
|RNA Binding Columns||25 szt.||2 x 50 szt||2-8°C|