Tick RNA/DNA Purification Kit

cat. no. E3590

GeneMATRIX Tick RNA/DNA Purification Kit is designed for simultaneous isolation of viral and bacterial RNA and DNA that live in small arachnids or insects.

The kit is optimized for the isolation of RNA and DNA from microorganisms (bacteria, viruses, fungi) that live in small (up to 5 mm long) arachnids or insects. A simple protocol enables a fast isolation. The first step is the efficient fragmentation of the arachnid/insect with the use of Tissue Grinding Tool. Then the material is lysed and filtrated. The addition of carrier RNA supports the binding of even small amounts of viral and bacterial nucleic acids. During short centrifugation RNA / DNA binds to the membrane. Unbound impurities remain in the column flow-through.

Traces of contaminants remaining on the membrane are efficiently removed in two wash steps. The elution of purified RNA / DNA is carried out with RNase-free water. Purified nucleic acids have a length of over 25 nt (miRNA). Purified nucleic acids are free of proteins, nucleases, and other impurities and are ready for use in amplification reactions or storage at -20°C.

Kit content

Content 25 preps
Buffer A 0.75 ml 15-25°C
Sol V 6.5 ml 15-25°C
Wash V1 15 ml 15-25°C
Wash RBW 15 ml 15-25°C
Proteinase K (20 mg/ml) 0.6 ml –20°C
RNase-free water 4.5 ml 15-25°C
Carrier RNA 150 μg* 15-25°C
DNA/RNA Binding Columns 25 szt. 15-25°C
Filtration Spin Columns 25 szt. 15-25°C
Tissue Grinding Tool 25 szt. 15-25°C
Protocol 1  

* Add 150 μl RNase free water to the tube containing 150 μg lyophilized carrier RNA to obtain a solution of 1 μg/μl.