GeneMATRIX Viral RNA/DNA Purification Kit is designed for simultaneous isolation of viral RNA and DNA. The procedure is suitable for use with plasma, serum, and other cell-free body fluids. Samples can be either fresh or frozen.
The procedure is optimized for use with a starting volume of 200 μl. A simple protocol based on four steps (lysis, binding, washing and elution) minimizes the time of isolation and the possibility of contamination of samples. The addition of carrier RNA supports the binding of even small amounts of viral nucleic acids. The elution of purified RNA / DNA is carried out with RNase-free water. Effectively purified are nucleic acids with a length of over 200 nt. Isolation of shorter fragments is possible, however, they are isolated with reduced efficiency. Purified nucleic acids are free of proteins, nucleases, and other impurities and are ready for use in amplification reactions or storage at -20°C.