GeneMATRIX FFPE RNA Purification Kit

cat. no. E3593

GeneMATRIX FFPE RNA Purification Kit is designed for rapid purification of total RNA enriched with small RNA molecules, shorter than 200 nucleotides from formalin-fixed, paraffin-embedded tissue sections. Firstly, all paraffin is removed from FFPE tissue sections by using one of deparaffinization method. Next, samples are incubated in an optimized lysis buffer, which contains Proteinase K, to release RNA from the sections. A short incubation at a higher temperature partially reverses formalin crosslinking of the released nucleic acids, improving RNA yield and quality as well as RNA performance in downstream enzymatic assays. The lysate is then transferred to specially designed mini spin-columns. These mini spin columns bind nucleic acids, remove impurities and prepare the samples for digestion with DNase I, which in the next stage effectively eliminates DNA. Addition of ethanol creates the proper conditions for selective binding of RNA to the membrane  GeneMATRIX. Following adjustment of binding conditions, the samples are applied to RNA binding spin-columns. All RNA molecules are adsorbed to the column matrix and contaminants are efficiently washed away. High-quality RNA is then eluted in RNase-free water. Special columns containing membranes with improved binding performance allows to reduce the elution volume and finally increase concentration of RNA. The maximum binding capacity of columns is up to 100 μg total RNA. Isolated RNA is ready for downstream applications without the need for ethanol precipitation.