KHV qPCR Kit is intended to detect koi herpesvirus (cyprinid herpesvirus 3) DNA in koi carp and common carp samples.
The purified viral DNA is amplified using real-time PCR and detected using a FAM-labeled probe. The virus is identified based on a highly conserved region in ORF 89 of the KHV genome. Additionally, the kit contains an endogenous internal control consisting of primers and a probe labeled with HEX dye for the carp glucokinase fragment. The internal control allows both extraction and amplification to be monitored.