Agarase from Streptomyces sp., β-agarase cleaves β 1-4 bonds in agarose, yielding soluble oligosaccharide multimers of neoagarobiose, thus allowing for a simple, quantitative recovery of intact nucleic acids from agarose gels.
Description:
- Digests the polysaccharide backbone of agarose yielding ethanol soluble oligosaccharides. The resulting carbohydrate molecules no longer gel or interfere with subsequent DNA manipulations.
- Allows for simple, quantitative recovery of intact nucleic acids from agarose gels.
- Suitable for purification of various DNA fragments ranging in size from large (>50 kb) down to small (<50 kb) one.
- Ideal for quantitative recovery of high molecular weight DNA from low-melting agarose gels.
- Can be heat inactivated (2 min at 95oC or 15 min at 65oC)
- Compatible with resin-based DNA purification schemes (i.e. EURx E3520).
