One Buffer - EURx

NR – buffer is not recommended, to see recommended buffer check product specifications; NA – heat inactivation is not active;
* – enzyme exhibits star activity under certain conditions; ^LS – reduced star activity (low star)

Enzyme	Cat.No.	Prototype	Star activity	Specificity	Buffer		Reaction temp. [ºC]	Inactivation temp. [ºC]	Assayed on
Enzyme	Cat.No.	Prototype	Star activity	Specificity	ONE	Specific	Reaction temp. [ºC]	Inactivation temp. [ºC]	Assayed on
AccI	E2004	AccI		GT^MKAC	100%	–	37	80	λ DNA
AcvI	E2005	PmaCI		CAC^GTG	100%	–	37	80	λ DNA/HindIII
AluI	E2010	AluI		AG^CT	100%	–	37	65	λ DNA
ApaI	E2015	ApaI		GGGCC^C	100%	–	25	65	pXba
AvaI	E2006	AvaI	*	C^YCGRG	100%	–	37	80	λ DNA
AvaII	E2007	AvaII	*	G^GWCC	100%	–	37	65	λ DNA
BalI	E2008	BalI		TGG^CCA	100%	–	37	65	λ DNA
BamHI	E2050	BamHI	*	G^GATCC	100%	–	37	NA	λ DNA
BanII	E2060	HgiJII	*	GRGCY^C	100%	–	37	65	λ DNA
BglI	E2080	BglI	*	GCCN₄^NGGC	100%	–	37	65	λ DNA
BglII	E2090	BglII		A^GATCT	100%	–	37	NA	λ DNA
BsiHKCI	E2109	AvaI	*	C^YCGRG	100%	–	65	NA	λ DNA
BssHII	E2115	BsePI	*	G^CGCGC	100%	–	50	80	λ DNA
BstXI	E2118	BstXI	*	CCAN₅^NTGG	25%	100%	50	65	λ DNA
ClaI	E2110	ClaI		AT^CGAT	100%	–	37	65	λ DNA
CviJI	E2125	CviJI	*	RG^CY	NR	100%	37	65	pBR322
CviJI*	E2126	CviJI*	*	G^C	NR	100%	37	65	pBR322
DdeI	E2140	DdeI		C^TNAG	100%	–	37	65	λ DNA
DpnI	E2135	DpnI		G^6mA^TC	100%	–	37	80	pBR322
DraI	E2145	AhaIII		TTT^AAA	100%	–	37	65	λ DNA
EcoRI	E2150	EcoRI	*	G^AATTC	100%	–	37	65	λ DNA
EcoRV	E2160	EcoRV	*	GAT^ATC	100%	–	37	80	λ DNA
FokI	E2170	FokI	*	GGATG(9/13)^	100%	–	37	65	λ DNA
HaeIII	E2180	HaeIII	*	GG^CC	100%	–	37	80	λ DNA
HhaI	E2190	HhaI	*	GCG^C	100%	–	37	65	λ DNA
HincII	E2200	HindII		GTY^RAC	100%	–	37	65	λ DNA
HindIII	E2220	HindIII	*	A^AGCTT	100%	–	37	65	λ DNA
HinfI	E2240	HinfI	*	G^ANTC	100%	–	37	80	λ DNA
HpaI	E2250	HpaI	*	GTT^AAC	100%	–	37	NA	λ DNA
HpaII	E2260	HpaII		C^CGG	100%	–	37	65	λ DNA
KpnI	E2280	KpnI	*	GGTAC^C	100%	–	37	NA	pXba
MboI	E2283	MboI		^GATC	100%	–	37	65	unmethylated λ DNA
MboII	E2284	MboII	*	GAAGA (8/7)^	100%	–	37	65	unmethylated λ DNA
MluI^LS	E2287	MluI		A^CGCGT	100%	–	37	65	λ DNA
MnlI	E2289	MnlI		CCTC (7/6)^	100%	–	37	65	λ DNA
MspI	E2290	HpaII		C^CGG	100%	–	37	65	λ DNA
NarI	E2291	NarI		GG^CGCC	100%	–	37	65	pXba
NcoI	E2292	NcoI	*	C^CATGG	100%	–	37	65	λ DNA
NdeI	E2293	NdeI	*	CA^TATG	100%	–	37	65	λ DNA
NheI	E2294	NheI	*	G^CTAGC	100%	–	37	65	λ DNA/HindIII
NotI^LS	E2295	NotI		GC^GGCCGC	100%	–	37	65	pBC4
NruI	E2297	NruI		TCG^CGA	100%	–	37	65	unmethylated λ DNA
NsiI	E2298	NsiI		ATGCA^T	100%	–	37	65	λ DNA
PinAI	E2299	AgeI		A^CCGGT	100%	–	37	65	λ DNA
PstI	E2300	PstI	*	CTGCA^G	100%	–	37	80	λ DNA
PvuI	E2320	PvuI	*	CGAT^CG	100%	–	37	80	pXba
PvuII^LS	E2330	PvuII		CAG^CTG	100%	–	37	NA	λ DNA
RsaI	E2340	RsaI		GT^AC	100%	–	37	65	λ DNA
RsrII	E2342	RsrII		CG^GWCCG	100%	–	37	65	λ DNA
SacI	E2350	SacI	*	GAGCT^C	100%	–	37	65	λ DNA/HindIII
SacII	E2352	SacII		CCGC^GG	100%	–	37	65	pXba
SalI^LS	E2370	SalI		G^TCGAC	100%	–	37	65	pBC4
Sau3AI	E2375	MboI	*	^GATC	NR	100%	37	65	unmethylated λ DNA
ScaI^LS	E2378	ScaI		AGT^ACT	100%	–	37	80	λ DNA
SfiI	E2380	SfiI	*	GGCCN₄^NGGCC	100%	–	50	NA	pBC4
SmaI	E2390	SmaI	*	CCC^GGG	100%	–	25	65	λ DNA/HindIII
SpeI	E2398	SpeI	*	A^CTAGT	100%	–	37	65	pXba
SphI	E2400	SphI	*	GCATG^C	100%	–	37	65	λ DNA
SspI	E2402	SspI	*	AAT^ATT	100%	–	37	65	λ DNA
StuI	E2405	StuI		AGG^CCT	100%	–	37	65	unmethylated λ DNA
TaqI	E2410	TaqI	*	T^CGA	100%	–	65	NA	unmethylated λ DNA
TaqII	E2411	TaqII	*	GACCGA (11/9)^	100%	–	65	NA	pBR322
TspDTI	E2502	TspDTI	*	ATGAA (11/9)^	NR	100%	70	NA	pUC19
TspGWI	E2501	TspGWI	*	ACGGA (11/9)^	NR	100%	70	NA	pBR322
Tth111I	E2420	Tth111I	*	GACN^NNGTC	100%	–	65	NA	pBC4
XbaI	E2430	XbaI	*	T^CTAGA	100%	–	37	65	λ DNA/HindIII
XhoI	E2440	XhoI		C^TCGAG	100%	–	37	65	λ DNA/HindIII