Description

• SNP Probe PCR Master Mix (2x) is designed for an accurate genotyping analysis of DNA samples.
• The master mix enables detection of gene mutations, SNPs and identification of microsatellite loci.
• SNP Probe PCR Master Mix (2x) is compatible with commercially available SNP genotyping assays as well as user-developed assays based on dual-labeled probes (TaqMan MGB, TaqMan, LNA, ZNA).
• The master mix contains onTaq DNA Polymerase, optimized reaction buffer, dNTPs (dATP, dCTP, dGTP, dTTP of ultrapure quality) and ROX passive reference dye.
• onTaq DNA Polymerase is a modified “hot start” enzyme which provides very tight inhibition of the polymerase activity at moderate temperatures and allows room temperature reaction setup.
• The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 95^oC for 15 minutes.
• Use of the “hot start” enzyme prevents extension of misprimed products and primer-dimers during reaction setup leading to higher specificity and sensitivity of PCR reactions.
• The passive ROX reference dye included in the master mix enables fluorescence normalization in particular cyclers. The use of ROX dye is required for all Applied Biosystems real-time PCR cyclers and optional for Agilent cyclers. ROX compensates for differences in fluorescence signal between wells caused by small differences in reaction volume and fluorescence fluctuations. ROX is not involved in the PCR reaction and does not interfere with real-time PCR on any instrument.