Polar-BAP

cat. no. E1027

Thermosensitive Bacterial Alkaline Phosphatase catalyzes the release of 5′- and 3′-phosphate groups from DNA, RNA, and nucleotides. Removes 5´ phosphates from DNA, RNA, rNTPs and dNTPs. Removes 5′-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures. The enzyme is resistant to chemical changes and active over a broad range of buffer conditions. It can be easily heat inactivated within 5 minutes at 70°C. Can be used to remove 5′-phosphates from DNA or RNA prior to 5′-end labeling. Ideal for diagnostic immunoassays and immunodetection of proteins and nucleic acids following blotting experiments. Used for protein dephosphorylation.