Source: Neisseria mucosa heidelbergensis

5’-G C T A G C-3’
3’-C G A T C G-5’

Recombinant. Purified from E.coli strain carrying the NheI gene from Neisseria nucosa heidelbergensis.

Reaction Temperature: 37°C

Prototype: NheI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest) in 1 hr in a total reaction volume of 50 µl.

Notes: 
– due to star activity it is not recommended to incubate over 4 hours or to use more than 100 units per reaction;
– salt concentration above 50 mM inhibits NheI activity.

Storage Conditions: Store at –20°C.

Quality Control:
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, and nonspecific single- and double-stranded DNase activities.