Source: Neisseria mucosa heidelbergensis
5’-G C T A G C-3’
3’-C G A T C G-5’
Recombinant. Purified from E.coli strain carrying the NheI gene from Neisseria nucosa heidelbergensis.
Reaction Temperature: 37°C
Inactivation Temperature (20 min): 65°C
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hr in a total reaction volume of 50 µl.
– due to star activity it is not recommended to incubate over 4 hours or to use more than 100 units per reaction;
– salt concentration above 50 mM inhibits NheI activity.
Storage Conditions: Store at –20°C.
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, and nonspecific single- and double-stranded DNase activities.