Source: Thermus aquaticus
5’-G A C C G A (N)11–3’
3’-C T G G C T (N) 9 -5’
Purified from E.coli strain that carries the cloned taqRII gene from Thermus aquaticus*.
* patent pending
Reaction Temperature: 65°C
Inactivation Temperature (20 min): —
1x ONE Buffer
Note 2: over 1 hr digestion is higly recommended. Best results are obtained with 3 hr digestion.
Unit Definition: One unit is the amount of enzyme required to digest 1 µg of pBR322 DNA to obtain stable digestion pattern in 1 hr in a total reaction volume of 50 µl.
Storage Conditions: Store at –20°C.
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.