tiTaq DNA Polymerase is a new generation “hot start” enzyme that is blocked by a biological component at moderate temperatures and allows room temperature reaction setup. The polymerase activity is restored during normal cycling conditions. Use of tiTaq DNA Polymerase allows for the increase of PCR specificity, sensitivity and yield in comparison to the conventional PCR assembly method. Automatic “hot start” PCR is a fast and convenient method when assembling multiple PCR reactions. Both increased specificity and reduced mispriming improve multiplex PCR. Thermostable tiTaq DNA Polymerase replicates DNA at 72°C and exhibits a half-life of 40 min at 95°C. tiTaq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.