RNase H (Ribonuclease H) is an RNA-specific endonuclease that cleaves the RNA of RNA/DNA hybrids. A minimum of four continuous base pairs of RNA/DNA hybrids is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. The enzyme does not degrade single-stranded nucleic acids, double-stranded DNA or double-stranded RNA.
Related products
DNase I (RNase-free)
cat. no. E1345
DNase I, nonspecific deoxyribonuclease that degrades both double-stranded and single-stranded DNA, endonucleolytically releasing 5′-phosphorylated di-, tri-, and oligonucleotide products. DNase I is used to remove template DNA following in vitro transcription, to remove contaminating DNA in total RNA preparations, to prepare DNA–free RNA prior to RT-PCR and RT-qPCR.
Exonuclease I
cat. no. E1150
Exonuclease I, catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5′ direction. Does not degrade dsDNA or RNA. Active under a wide variety of buffer conditions. Exonuclease I is ideal for: removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis, removal of single-stranded primers for nested PCR reactions, […]
Exonuclease III (E.coli)
cat. no. E1140
Exonuclease III catalyzes the removal of mononucleotides from dsDNA starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs of less than 4 bases, yielding nucleoside 5´-phosphates. The enzyme contains DNA 3′-phosphatase, hydrolyzing 3′-terminal phosphomonoesters and AP endonuclease, cleaving phosphodiester bonds at apurinic or apyrimidinic sites to produce 5′-termini that are base-free deoxyribose […]
Exonuclease T5
cat. no. E1130
T5 Exonuclease is a product of T5 phage D15 gene derived from E. coli. T5 exonuclease degrades DNA in a 5’→3′ direction and also exhibits endonucleolytic activity on flap structures (branched duplex DNA containing a free single-stranded 5′-end). T5 exonuclease has been shown to have no effect on double stranded circular DNA.
Exoribonuclease XRN-1
cat. no. E1155
XRN-1 is highly processive 5´→3´ exoribonuclease, requiring 5´ monophosphate. Description: XRN-1 also acts on 5´ monophosphate ssDNA with greatly reduced efficiency. XRN-1 is not efficient in removing RNAs with recessed 5´P, like tRNA. It does not cleave dsDNA, ssDNA and RNA which contain di-, triphosphate, OH and capped 5´ ends.
Lambda Exonuclease
cat. no. E1180
Lambda Exonuclease, double-stranded specific DNase produced by Escherichia coli upon lambda bacteriophage infection. Lambda exonuclease processively degrades one strand of double-stranded DNA (dsDNA) in the 5’→3′ direction. The preferred substrate is blunt-ended, 5´-phosphorylated dsDNA. Lambda exonuclease has reduced activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini.
