5′-A C G C G T-3′

3′-T G C G C A-5′

Reaction Temperature: 37°C

Inactivation Temperature (20 min): 65°C

Prototype: MluI

Source: Micrococcus luteus

Package Contents:

• MluI RS*
• 10 x ONE Buffer
• BSA [100x]

Added as separate component to prevent reaction buffer precipitation.

• Dilution Buffer # 1

Added for enzymes exceeding 10 U/µl in concentration. High protein concentration warrants optimal stability during prolonged storage. Use dilution buffer to prepare short term working stocks (510 U/µl, non-freezing at -20°C).

Storage Conditions: Store at -20°C.

Double Digestion – Buffer Compatibility:

ONE Buffer is compatible with most EURx restriction enzymes.

Restriction Enzyme Buffer Compatibility:

Both, enzyme and buffers are fully compatible to restrictases and buffer systems from other manufacturers and can be used along in double digestions. To obtain best results, consult the corresponding manuals of all involved products.

DNA Methylation:

No Inhibition: dam, dcm

Potential inhibition: EcoKI

Inhibition (Blocked): CpG