onHybrid PCR Master Mix (2x)

nr kat. E2755

Description:

  • onHybrid PCR Master Mix (2x) is a ready-to-use solution containing onHybrid DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs.
  • Use of onHybrid PCR Master Mix (2x) allows to save time and reduce
    contamination risk due to fewer pipetting steps during PCR reaction setup.
  • onHybrid DNA Polymerase contains a modified „hot start” enzyme that is blocked at moderate temperatures and allows room temperature reactions setup.
  • The polymerase activity is restored during 10 min initial denaturation step.
  • Use of onHybrid DNA Polymerase allows for the increase of PCR specificity, sensitivity and yield in comparison to the conventional PCR assembly method.
  • onHybrid is a genetically engineered thermophilic DNA polymerase.
  • Ultrapure recombinant enzyme.
  • The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5’→3′ direction in the presence of magnesium ions.
  • The enzyme exhibits the 3’→5′ proofreading activity, resulting in 50-fold higher PCR fidelity than possible with Taq DNA Polymerases.
  • The enzyme generates blunt ends.
  • Enhanced polymerase processivity allows to use shorter extension times.
  • The modification of onHybrid DNA Polymerase enhances target length capability of PfuPlus! DNA Polymerase with regard to genomic targets (up to 10 kb from human genomic DNA).
  • Due to the polymerase modification, the optimal reaction conditions (especially annealing temperatures) differ from standard PCR protocols.
  • onHybrid PCR Master Mix (2x) is recommended for general use in PCR, use in high-fidelity PCR, PCR of GC-rich sequences