GeneMATRIX Food-Extract DNA Purification Kit is designed for rapid purification of DNA from raw or processed food of plant, animal or mixed origin and can be used for the identification of DNA from Genetically Modified Organisms (GMO) in the food. Purified DNA is free of contaminants, such as: proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others.
Sample is finely grinded and remaining tissue- and cellular structures are subsequently solubilized by lysis in the presence of special desintegrating buffer, which preserves integrity and stimulates quantitative recovery of all traces of DNA. Further, Proteinase K digests contaminating proteins, including stripping-off DNA of all bound proteins, among them nucleases. Specialized solution is added to precipitate enzyme inhibitors that strongly inhibit downstream applications. Optimized buffer and ethanol provide selective conditions for DNA binding to the DNA binding spin-columns. Contaminants remaining on the resin are efficiently removed in two wash steps. High-quality cellular DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE. Isolated DNA is ready for downstream applications without the need for the ethanol precipitation.