onPfuPlus! DNA Polymerase

nr kat. E1113

Extremely thermostable hot start proofreading DNA polymerase blend, formulated for efficient site-directed mutagenesis and synthesis of DNA products up to 20 kb.

Description:

  • onPfuPlus! DNA Polymerase is a modified hot start enzyme that is blocked at moderate temperatures and allows room temperature reaction setup.
  • The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 95°C for 10 minutes.
  • Use of onPfuPlus! DNA Polymerase allows for the increase of PCR specificity, sensitivity and yield in comparison to the conventional PCR assembly method.
  • onPfuPlus! is a modified and optimized hyperthermophilic Pfu DNA Polymerase blended with the thermostable polymerase-enhancing factor.
  • Ultrapure recombinant enzyme.
  • The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5′3′ direction in the presence of magnesium ions.
  • The enzyme exhibits the 3′5′ proofreading activity, resulting in over 10-fold higher PCR fidelity than possible with Taq DNA Polymerases.
  • The constituent of onPfuPlus! DNA Polymerase, the polymerase-enhancing factor, enhances PCR product yields and increases target length capability of Pfu DNA Polymerase.
  • onPfuPlus! DNA Polymerase is recommended for use in high-fidelity PCR, PCR of GC-rich sequences or problematic secondary structures, site-directed mutagenesis and cloning of blunt-ended PCR products.
  • The enzyme is also recommended for general use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of PCR products up to 20 kb.