GeneMATRIX Soil DNA Purification Kit is designed for the rapid isolation of highly pure, humic-free microbial DNA from environmental samples: soil, sediment, compost, manure and filtered water samples, etc. Purified DNA is free of contaminants, such as: humic substances, proteins, lipids, dyes, detergents, buffers, salts, divalent cations, etc.
Soil sample is added to a bead beating tube containing beads and lysis solution. The principal is to lyse the microorganisms in the soil by a combination of heat, detergent and mechanical force against the beads. Specialized solution is added to precipitate humic substances that strongly inhibit downstream applications. Optimized buffer and ethanol provide selective conditions for DNA binding to the DNA binding spin-columns. Contaminants remaining on the resin are efficiently removed in two wash steps. High-quality cellular DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE. Isolated DNA is ready for downstream applications without the need for the ethanol precipitation.