Description:

• NXT tiTaq PCR Kit is designed for fast-cycling PCR on any thermal cycler.
• NXT tiTaq PCR Kit shortens PCR cycling time without affecting the yield and PCR performance.
• The annealing and extension steps require 5 s and 3 s per 100 bp respectively.
• PCR does not require redesigning of primers.
• NXT tiTaq PCR Kit is a ready-to-use solution containing hot start NXT tiTaq DNA Polymerase, reaction buffer, MgCl₂ and dNTPs.
• NXT tiTaq DNA Polymerase contains a new generation „hot start” enzyme that is blocked at moderate temperatures and allows room temperature reactions setup.
• The polymerase activity is restored during normal cycling conditions.
• “Hot start” PCR may increase specificity, sensitivity and yield of a PCR reaction in comparison to the conventional PCR assembly method.
• NXT tiTaq DNA Polymerase replicates DNA at 72°C and exhibits a half-life of 40 min at 95°C.
• Contains the 5′→3′ exonuclease activity.
• Lacks the 3′→5′ exonuclease activity.
• Adds extra A at the 3′ ends.
• NXT tiTaq PCR Kit is supplied with 10 x Color Load solution which allows for direct loading PCR reactions on the gel.
• NXT tiTaq PCR Kit allows to obtain a wide range of PCR products up to 4 kb and 10 kb from complex genomic or episomal DNA respectively.