Opti Bst DNA Polymerase LF

nr kat. E1079

Large exonuclease 5’→3’ free fragment of thermophilic Bst DNA Polymerase with strand displacement activity.

Description:

  • Opti Bst DNA Polymerase is a moderately thermostable enzyme from Bacillus stearothermophilus genetically optimized for faster amplification and more flexible reaction conditions.
  • Optimized for LAMP (Loop Medieted Isothermal Amplification)
  • Enhanced dUTP incorporation compared to Bst DNA Polymerase.
  • Ultrapure, recombinant protein.
  • The enzyme replicates DNA optimally at 65oC.
  • Catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium ions.
  • Lacks the 5´→3´ exonuclease activity, while retaining the polymerase activity (1).
  • Broad activity range; can replace mezophilic polymerases as well as synthesize DNA at high temperatures. Thus it is suitable for amplification of difficult DNA templates, including repetitive sequences, GC-rich regions and problematic secondary structures (2, 3).
  • Can be heat inactivated at temperatures above 80°C.
  • Active over wide range of reaction buffer conditions and magnesium ions concentrations.
  • Used in isothermal DNA sequencing at elevated temperatures.
  • Ideal for DNA synthesis reactions requiring strand displacement.